We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
糙皮侧耳菌丝胞内钙离子参与高温响应及活性氧调控.
- Authors
卢树卓; 药欣荣; 黄晨阳; 郑素月; 邬向丽
- Abstract
Mycelia of Pleurotus ostreatus were treated with high temperature(40 ℃) and then observed for mycelial growth and intracellular calcium ion content in response to high temperature. Then different calcium ion inhibitors and chelators, including BAPTA-AM(intracellular calcium ion chelators), TFP(calmodulin inhibitor), LaCl3(plasma membrane calcium channel inhibitor), EGTA(extracellular calcium ion chelating agent), and heparin(IP3 receptor antagonist) were added to the medium, respectively, to investigate effects of intracellular calcium ions from different sources on mycelial growth and ROS level under heat stress. The results showed that high temperature inhibited mycelial growth and resulted in an increase in intracellular calcium ion content. Under high temperature, addition of BAPTA-AM, EGTA or TFP resulted in a significantly higher mycelial inhibition rate compared with that of the heat stressed mycelia without any drug treatment, suggesting that mycelias recovery after heat stress may be promoted by increased intracellular calcium ions from intracellular calcium pool release, extracellular calcium inflow, and cytoplasmic calcium release induced by binding calmodulin. When no calcium ion inhibitor or chelator was added, high temperature resulted in a significant increase in ROS level. In contrast, with the presence of EGTA, TFP or heparin, ROS level decreased under high temperature. This suggested that calcium ions from extracellular calcium inflow, cytoplasmic calcium release induced by binding calmodulin, and IP3 receptor channels may regulate ROS signalling molecules and promote ROS accumulation under heat stress.
- Subjects
CALCIUM ions; INTRACELLULAR calcium; PLEUROTUS ostreatus; CALMODULIN; CELL membranes; HIGH temperatures; CALCIUM channels; CHELATING agents
- Publication
Acta Edulis Fungi, 2021, Vol 28, Issue 6, p31
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2021.06.005