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- Title
Comparison of Real-time Polymerase Chain Reaction Results by Different Illumination Methods.
- Authors
Ji-Soo Hwang; Jong-Dae Kim; Yu-Seop Kim; Hye-Jeong Song; Chan-Young Park
- Abstract
In this paper, we introduce the diagonal illumination method and the vertical illumination method of the real-time polymerase chain reaction (PCR) system and compare the fluorescence intensity change patterns of the two methods using images captured with a digital single lens reflex (DSLR) camera during the PCR process. To overcome the problems of diagonal illumination, the PCR was performed by vertical illumination. The results of the PCR process with the proposed vertical illumination method were similar to those of the diagonal illumination method.
- Subjects
FLUORESCENCE; POLYMERASE chain reaction; FIBER optics; DIGITAL single-lens reflex cameras; LIGHTING
- Publication
Sensors & Materials, 2018, Vol 30, Issue 3,Part 1, p397
- ISSN
0914-4935
- Publication type
Article
- DOI
10.18494/SAM.2018.1760