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- Title
AIBP 基因表达下调促进心肌微血管内皮细胞血管新生.
- Authors
马晓磊; 吕安林; 姜晓宇; 邱翠婷; 李珊; 郭显
- Abstract
Objective: To explore the influence of knockdown the high expressed gene AIBP by RNA interference on angiogenesis in cardiac microvascular endothelial cells (CMEC). Methods: CMECs was isolated from rats with collagenase digestion, the transfected group, blank control group and negative control group were included. The transfected efficiency was detected by RT-PCR. The effect of AIBP-knockdown on angiogenesis on cell proliferation was analyzed by CCK-8 assay, cell migration was assessed by using migration assays and cell's angiogenic activity was studied by tube formation experiment. Results: The lentivirus vector infected the CMECs steadily and efficiently. Compared with the control groups, the expression of AIBP of transfected group was significantly decreased (P<0.01). The cell proliferation of transfected group was enhanced (P<0.01), cell migration and angiogenesis of transfected group was promoted (P<0.01). Conclusions: Knockdown the high expressed gene AIBP can significantly enhance CMECs proliferation, promote CMECs migration and angiogenesis.
- Publication
Progress in Modern Biomedicine, 2015, Vol 15, Issue 22, p4230
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2015.22.008