We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality.
- Authors
Yun-Zhu Guo; Li-Hua Sun; Oberthuer, Dominik; Chen-Yan Zhang; Jian-Yu Shi; Jiang-Lei Di; Bao-Liang Zhang; Hui-Ling Cao; Yong-Ming Liu; Jian Li; Qian Wang; Huan-Huan Huang; Jun Liu; Schulz, Jan-Mirco; Qiu-Yu Zhang; Jian-Lin Zhao; Betzel, Christian; Jian-Hua He; Da-Chuan Yin
- Abstract
High-quality protein crystals of suitable size are an important prerequisite for applying X-ray crystallography to determine the 3-dimensional structure of proteins. However, it is often difficult to obtain protein crystals of appropriate size and quality because nucleation and growth processes can be unsuccessful. Here, we show that by adsorbing proteins onto porous polystyrene-divinylbenzene microspheres (SDB) floating on the surface of the crystallisation solution, a localised high supersaturation region at the surface of the microspheres and a low supersaturation region below the microspheres can coexist in a single solution. The crystals will easily nucleate in the region of high supersaturation, but when they grow to a certain size, they will sediment to the region of low supersaturation and continue to grow. In this way, the probability of crystallisation and crystal quality can be simultaneously increased in a single solution without changing other crystallisation parameters.
- Subjects
CRYSTALLIZATION; X-ray crystallography; PROTEIN structure; DISCONTINUOUS precipitation; SUPERSATURATION
- Publication
Scientific Reports, 2014, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep07308