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- Title
A translational regulator MHZ9 modulates ethylene signaling in rice.
- Authors
Huang, Yi-Hua; Han, Jia-Qi; Ma, Biao; Cao, Wu-Qiang; Li, Xin-Kai; Xiong, Qing; Zhao, He; Zhao, Rui; Zhang, Xun; Zhou, Yang; Wei, Wei; Tao, Jian-Jun; Zhang, Wan-Ke; Qian, Wenfeng; Chen, Shou-Yi; Yang, Chao; Yin, Cui-Cui; Zhang, Jin-Song
- Abstract
Ethylene plays essential roles in rice growth, development and stress adaptation. Translational control of ethylene signaling remains unclear in rice. Here, through analysis of an ethylene-response mutant mhz9, we identified a glycine-tyrosine-phenylalanine (GYF) domain protein MHZ9, which positively regulates ethylene signaling at translational level in rice. MHZ9 is localized in RNA processing bodies. The C-terminal domain of MHZ9 interacts with OsEIN2, a central regulator of rice ethylene signaling, and the N-terminal domain directly binds to the OsEBF1/2 mRNAs for translational inhibition, allowing accumulation of transcription factor OsEIL1 to activate the downstream signaling. RNA-IP seq and CLIP-seq analyses reveal that MHZ9 associates with hundreds of RNAs. Ribo-seq analysis indicates that MHZ9 is required for the regulation of ~ 90% of genes translationally affected by ethylene. Our study identifies a translational regulator MHZ9, which mediates translational regulation of genes in response to ethylene, facilitating stress adaptation and trait improvement in rice. The authors identify a GYF domain-containing protein MHZ9 in rice, which regulates ethylene signaling by directly binding to OsEBF1/2 and other mRNAs thus regulating their translation efficiency in P-body via interacting with OsEIN2.
- Subjects
ETHYLENE; PROTEIN domains; GENETIC regulation; TRANSCRIPTION factors; BRASSINOSTEROIDS
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-40429-0