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- Title
Isatis tinctoria L. Leaf Extract Inhibits Replicative Senescence in Dermal Fibroblasts by Regulating mTOR-NF-κB-SASP Signaling.
- Authors
Woo, Jieun; Shin, Seoungwoo; Ji, Hyanggi; Ryu, Dehun; Cho, Eunae; Kim, Youngseok; Kim, Junoh; Park, Deokhoon; Jung, Eunsun
- Abstract
Senescent fibroblasts progressively deteriorate the functional properties of skin tissue. Senescent cells secrete senescence-associated secretory phenotype (SASP) factor, which causes the aging of surrounding non-senescent cells and accelerates aging in the individuals. Recent findings suggested the senomorphic targeting of the SASP regulation as a new generation of effective therapeutics. We investigated whether Isatis tinctoria L. leaf extract (ITE) inhibited senescence biomarkers p53, p21CDKN1A, and p16INK4A gene expression, and SASP secretions by inhibiting cellular senescence in the replicative senescent human dermal fibroblast (RS-HDF). ITE has been demonstrated to inhibit the secretion of SASP factors in several senomorphic types by regulating the MAPK/NF-κB pathway via its inhibitory effect on mTOR. ITE suppressed the inflammatory response by inhibiting mTOR, MAPK, and IκBα phosphorylation, and blocking the nuclear translocation of NF-κB. In addition, we observed that autophagy pathway was related to inhibitory effect of ITE on cellular senescence. From these results, we concluded that ITE can prevent and restore senescence by blocking the activation and secretion of senescence-related factors generated from RS-HDFs through mTOR-NF-κB regulation.
- Subjects
THERAPEUTICS; BIOMARKERS; MEDICINAL plants; FIBROBLASTS; GERIATRICS; AUTOPHAGY; CELL physiology; PHYTOCHEMICALS; CELLULAR signal transduction; CELLULAR aging; GENE expression; CELL proliferation; DESCRIPTIVE statistics; PLANT extracts; DATA analysis software; PHENOTYPES; PHOSPHORYLATION
- Publication
Nutrients, 2022, Vol 14, Issue 9, p1979
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu14091979