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- Title
Early Detection of Aβ Deposition in the 5xFAD Mouse by Amyloid PET.
- Authors
Oh, Se Jong; Lee, Hae-June; Kang, Kyung Jun; Han, Sang Jin; Lee, Yong Jin; Lee, Kyo Chul; Lim, Sang Moo; Chi, Dae Yoon; Kim, Kyeong Min; Park, Ji-Ae; Choi, Jae Yong
- Abstract
<italic>Purpose. </italic>18F-FC119S is a positron emission tomography (PET) tracer for imaging <italic>β</italic>-amyloid (A<italic>β</italic>) plaques in Alzheimer’s disease (AD). The aim of this study is to evaluate the efficacy of 18F-FC119S in quantitating A<italic>β</italic> deposition in a mouse model of early amyloid deposition (5xFAD) by PET.<italic> Method</italic>. Dynamic 18F-FC119S PET images were obtained in 5xFAD (n=5) and wild-type (WT) mice (n=7). The brain PET images were spatially normalized to the M. Mirrione T2-weighted mouse brain MR template, and the volumes of interest were then automatically drawn on the cortex, hippocampus, thalamus, and cerebellum. The specific binding of 18F-FC119S to A<italic>β</italic> was quantified as the distribution volume ratio using Logan graphical analysis with the cerebellum as a reference tissue. The A<italic>β</italic> levels in the brain were also confirmed by immunohistochemical analysis.<italic> Result</italic>. For the 5xFAD group, radioactivity levels in the cortex, the hippocampus, and the thalamus were higher than those for the WT group. In these regions, specific binding was approximately 1.2-fold higher in 5xFAD mice than in WT. Immunohistochemistry supported these findings; the 5xFAD showed severe A<italic>β</italic> deposition in the cortex and hippocampus in contrast to the WT group.<italic> Conclusion</italic>. These results demonstrated that 18F-FC119S PET can successfully distinguish A<italic>β</italic> depositions in 5xFAD mice from WT.
- Publication
Contrast Media & Molecular Imaging, 2018, p1
- ISSN
1555-4309
- Publication type
Article
- DOI
10.1155/2018/5272014