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- Title
TOXICITY EVALUATION BY FLOW CYTOMETRIC ANALYSIS OF NANOPARTICLES USING THE UNICELLULAR ALGA CHLORELLA.
- Authors
PETRESCU, Constantin-Marian; CALU, Larisa; DOBRE, Ana-Maria; TURCUS, Violeta; BRATOSIN, Daniela
- Abstract
The use of nanoparticles for biological and medical applications has rapidly increased and the potential for human and ecological toxicity is a growing area of investigation. For assessing cytotoxicity of nanoparticles we have attempted to assess by flow cytometry the toxicity and ecotoxicity of nanoparticles using the unicellular alga Chlorella fusca for developing an in vitro system that could provide an accurate, predictive early screen for assessing the toxicity of different pollutants. Consequently, to understand the mechanisms underlying the toxicity process of nanoparticles we analyzed by flow cytometry the morphological changes (light scattering properties), accompanied by optical and scanning microscopic observations. The investigation showed that analysis by flow cytometry in the system FSC / SSC of Chlorella cells incubated for variable in the presence of different dilutions of nanoparticles show induced morphological changes also observed by complementary microscopic techniques. Metabolic activity based on the level of esterases activity with Calcein-AM is a possibility to determine the toxicity of pollutants, including the study of toxicity of nanoparticles prior to their application in various fields of economic life, due to sensitivity and possibility to quantify the results.
- Subjects
FLOW cytometry; PSYCHODIAGNOSTICS; CHLORELLA; CHLORELLACEAE; CHLORELLA pyrenoidosa
- Publication
Studia Universitatis Vasile Goldis Seria Stiintele Vietii (Life Sciences Series), 2013, Vol 23, Issue 3, p381
- ISSN
1584-2363
- Publication type
Article