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- Title
Mechanism of tomato plants enhanced disease resistance against early blight primed by arbuscular mycorrhizal fungus Glomus versiforme.
- Authors
Song Yuan-Yuan; Lang Rui-Long; Wei Xiao-Chen; Lu Yong-Jian; Tang Zhao-Yang; Wu Guo-Zhao; Su Yi-Juan; Zeng Ren-Sen
- Abstract
Arbuscular mycorrhiza (AM) can not only improve host plants nutrient absorption, but also enhance their disease resistance. Taking the tomato (Lycopersicon esculentum) seedlings preinoculated with arbuscular mycorrhizal fungus (AMF) Glomus versiforme as test materials, this paper studied their protective enzyme activities and defense?related genes expression, and their resistance against a fungal pathogen Alternaria solani Sorauer which causes early blight. The seedlings preinoculated with AMF and later inoculated with A. solani showed significantly higher activities of superoxide dismutase (SOD) and peroxidase (POD) in leaves. The leaf SOD activity of the dually inoculated plants reached the maximum 18 h after pathogen inoculation, being 28.6%, 79.2%, and 82.8% higher than that of the plants with G. versiforme inoculation alone, pathogen inoculation alone, and non-inoculation, and the leaf POD activity reached the maximum 65 h after pathogen inoculation, being 762%, 18.3%, and 1710% higher, respectively. Real time RT-PCR analysis showed that dual inoculation with G. versiforme and A. solani strongly induced the expression of three defense-related genes. The transcript levels of pathogen-related protein (PR1), basic type &bgr;1,3-glucanase (PR-2), and chitinase (PR-3) in leaves were 9.67-, 8.54-, and 13.4-fold higher, as compared with the non-inoculation control, respectively. Bioassay showed that the disease incidence and disease index of the seedlings pre-inoculated with G. versiforme were reduced by 36.3%and 61.4%, respectively, as compared with the non-mycorrhizal control plants. These findings indicated that mycorrhizal colonization could induce stronger and quicker defense responses of host to mato plants, and priming could be an important mechanism of the enhanced disease resistance of mycorrhizal tomato plants.
- Subjects
TOMATO disease &; pest resistance; VESICULAR-arbuscular mycorrhizas; GLOMUS (Fungi); HOST plants; PLANT-fungus relationships; GENE expression in plants; REVERSE transcriptase polymerase chain reaction
- Publication
Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao, 2011, Vol 22, Issue 9, p2316
- ISSN
1001-9332
- Publication type
Article