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- Title
Evaluation of a solution chemiluminescent hybridization assay for identification of human papillomavirus from paraffin-embedded tissues.
- Authors
Didier, J. M.; Mougin, C.; Bettinger, D.; Vallet, A.; Bernard, C.; Kantelip, J. P.; Lab, M.
- Abstract
Aims To evaluate the use of the Digene Hybrid Capture™ system, a chemituminescent hybridization assay for the analysis of low and intermediate/high risk human papillomavirus (HPV) DNA, on paraffin-embedded tissues and to investigate possible reasons for reaction failure. Methods Fifty cervical biopsies were tested by an in situ hybridization (ISH) method using probes for HPV 6/11, 16/18, 31/33/51 and by the Digene Hybrid Capture%trade; system. Results The ISH was able to detect HPV in 23 out of the 50 biopsies. Eight samples were positive with the HPV 6/11 probe. 4 with the 16/18 probe. 6 with the 31/33/51 probe and 5 had mixed infections. With the Hybrid Capture™ system. having taken the mixed infections into account, 40 samples gave concordant results. while total and partial discordances were observed in 6 and 4 samples respectively. The relative sensitivity of the new assay was 91.6% and specificity 84.2% for low risk HPVs; and 80% and 94,3% respectively for intermediate/high risk HPVs. Agreement rates on HPV positivity for either low risk or intermediate/high risk types were 86% and 94% respectively in comparison with ISH results. Conclusion The Hybrid Capture™ system is simple. reliable in use and suitable for routine discrimination of HPVs in tissue sections from paraffin-embedded blocks. It might thus be of clinical value in the diagnosis and prognosis of HPV infection.
- Subjects
PAPILLOMAVIRUSES; CHEMILUMINESCENCE assay; DNA; DIAGNOSTIC use of in-situ hybridization; BIOLOGICAL assay
- Publication
Journal of the European Academy of Dermatology & Venereology, 1996, Vol 7, Issue 3, p240
- ISSN
0926-9959
- Publication type
Article
- DOI
10.1111/j.1468-3083.1996.tb00577.x