We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Homeoprotein Hbx4 represses the expression of the adhesion molecule DdCAD-1 governing cytokinesis and development
- Authors
Kim, Ji-Sun; Seo, Ji-Hui; Yim, Hyung-Soon; Kang, Sa-Ouk
- Abstract
Abstract: We investigated the function of homeodomain-containing protein Hbx4 in Dictyostelium discoideum. Hbx4-overexpressing cells (Hbx4OE) displayed defects in growth rate and cytokinesis and showed differences in slug motility and cell-type proportioning from KAx3. Furthermore, the overexpression of Hbx4 inhibited the induction of cadA, which encoded the Ca2+-dependent cell adhesion molecule DdCAD-1, despite expression of csaA and gpaB. The electrophoretic mobility shift assay showed that the promoter of cadA contained the Hbx4-binding site. Moreover, constitutively expressed DdCAD-1 in Hbx4OE rescued the defects in cytokinesis and development. These results suggest that Hbx4 modulates DdCAD-1-mediated cytokinesis and cell-type proportioning.
- Subjects
PROTEINS; GENE expression; CYTOKINESIS; DICTYOSTELIUM discoideum; CELL adhesion; CALCIUM; HOMEOBOX genes; ELECTROPHORESIS
- Publication
FEBS Letters, 2011, Vol 585, Issue 12, p1864
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.04.052