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- Title
Platycodin D inhibits platelet function and thrombus formation through inducing internalization of platelet glycoprotein receptors.
- Authors
Luo, Qi; Wei, Guangyu; Wu, Xiaoqing; Tang, Kai; Xu, Mengdi; Wu, Yulu; Liu, Yun; Li, Xiaoqian; Sun, Zengtian; Ju, Wen; Qi, Kunming; Chen, Chong; Yan, Zhiling; Cheng, Hai; Zhu, Feng; Li, Zhenyu; Zeng, Lingyu; Xu, Kailin; Qiao, Jianlin
- Abstract
<bold>Background: </bold>Platycodin D (PD) is one of the major bioactive components of the roots of Platycodon grandiflorum and possesses multiple biological and pharmacological properties, such as antiviral, anti-inflammatory, and anti-cancer activities. However, whether it affects platelet function remains unclear. This study aims to evaluate the role of PD in platelet function and thrombus formation.<bold>Methods: </bold>Platelets were treated with PD followed by measuring platelet aggregation, activation, spreading, clot retraction, expression of glycoprotein receptors. Moreover, mice platelets were treated with PD and infused into wild-type mice for analysis of in vivo hemostasis and arterial thrombosis.<bold>Results: </bold>Platycodin D treatment significantly inhibited platelet aggregation in response to collagen, ADP, arachidonic acid and epinephrine, reduced platelet P-selectin expression, integrin αIIbβ3 activation, spreading on fibrinogen as well as clot retraction, accompanied with decreased phosphorylation of Syk and PLCγ2 in collagen-related peptide or thrombin-stimulated platelets. Moreover, PD-treated mice platelets presented significantly impaired in vivo hemostasis and arterial thrombus formation. Interestingly, PD induced internalization of glycoprotein receptors αIIbβ3, GPIbα and GPVI. However, GM6001, cytochalasin D, BAPTA-AM and wortmannin did not prevent PD-induced internalization of receptors.<bold>Conclusions: </bold>Our study demonstrates that PD inhibits platelet aggregation, activation and impairs hemostasis and arterial thrombosis, suggesting it might be a potent anti-thrombotic drug.
- Subjects
SAPONINS; GLYCOPROTEIN receptors; BIOACTIVE compounds; PLANT roots; BLOOD platelets
- Publication
Journal of Translational Medicine, 2018, Vol 16, Issue 1, pN.PAG
- ISSN
1479-5876
- Publication type
journal article
- DOI
10.1186/s12967-018-1688-z