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- Title
manifests potent anti-proliferative activity on cultured Daudi cells.
- Authors
Parmar, Felisa; George, Linz-Buoy; Highland, Hyacinth; Parmar, Felisa P; Highland, Hyacinth N
- Abstract
<bold>Objective: </bold>The aim of this study is to observe the apoptosis of Phyllanthus fraternus Webster against Daudi cells and to study its primary mechanism.<bold>Materials and Methods: </bold>Antiproliferative activity of cultured Daudi cells was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay in a dose- and time-dependent manner after treatment with the hydroalcoholic extract of P. fraternus . Trypan blue viability assay was also performed. Apoptosis induction in the cells posttreatment was determined by DNA fragmentation assay, Agarose gel electrophoresis, and Acridine orange/Ethidium bromide dual staining. Protein isolation and analysis was carried out using the standard polyacrylamide gel electrophoresis protocols.<bold>Results: </bold>The extracts inhibited the growth and proliferation of Daudi cells through induced cell death, which was dose-dependent and time-dependent. The IC50 value was found to be 220 μg/ml after 72 h of treatment. The induction of DNA fragmentation and increase in a number of apoptotic cells posttreatment suggest the possibility of apoptosis induction. A significant decrease in protein level was also observed.<bold>Conclusion: </bold>The results raise the possibility that the hydroalcoholic extract of P. fraternus could be a potent chemotherapeutic agent for the treatment of various cancers. Further evaluation of its potency as a chemotherapeutic agent is imperative.
- Subjects
POLYACRYLAMIDE gel electrophoresis; PHYLLANTHUS; CELL death; ACRIDINE orange; CELLS; CELL physiology; ANTINEOPLASTIC agents; APOPTOSIS; PLANTS; TUMORS; PLANT extracts; CELL lines; PHARMACODYNAMICS
- Publication
Journal of Cancer Research & Therapeutics, 2020, Vol 16, Issue 1, p71
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/jcrt.JCRT_429_17