We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
β-Galactosidase Activity as a Biomarker of Replicative Senescence during the Course of Human Fibroblast Cultures.
- Authors
MAIER, ANDREA B.; WESTENDORP, RUDI G. J.; VAN HEEMST, DIANA
- Abstract
It has been called into question whether the commonly used β-galactosidase staining is a reliable biomarker of cellular senescence because induction of β-galactosidase activity also occurs independently of senescence. Here, we tested whether cytochemically detectable β-galactosidase activity is reproducible and reflects the rate of cellular aging in vitro. Therefore, we serially cultured fibroblasts from 12 different donors and stained the cells for β-galactosidase at pH 6 until the onset of the permanent growth arrest of the individual cultures. All fibroblast strains displayed a high replicative capacity with a similar growth pattern during the exponential growth phase and a very high interbiopsy variability in the onset of decreased mitotic activity and in the onset of growth arrest. Correspondingly, β-galactosidase activity was low during the exponential growth phase, with an individually defined significant increase in activity when the growth speed of the culture decreased. The increase in β-galactosidase activity was a better predictor for the onset of the decreased growth speed than the chronological life span of the culture expressed in population doublings. Within the phase of decreased mitotic activity, we observed a high fluctuation in the percentage of positively stained fibroblasts. Thus, our results support β-galactosidase activity as a reliable biomarker for the course of replicative senescence, if used under defined standardized conditions.
- Subjects
BETA-galactosidase; AGING; BIOMARKERS; STAINS &; staining (Microscopy); FIBROBLASTS; CONNECTIVE tissue cells
- Publication
Annals of the New York Academy of Sciences, 2007, Vol 1100, p323
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1395.035