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- Title
Advantages of a synchrotron light source for fluorescence‐detected linear dichroism.
- Authors
Jones, Nykola C.; Rodger, Alison; Hoffmann, Søren V.
- Abstract
Fluorescence‐detected linear dichroism (FD‐LD) enables one to collect linear dichroism spectra for oriented fluorophores in the presence of other absorbing species and light scattering. The experiment proceeds by scanning the excitation wavelength and using a filter to collect only emitted photons from the fluorophore. Thus, it has the potential to give data with enhanced selectivity and quality. By using a synchrotron radiation light source and fluorescence‐detection, we show data for a range of fluorophores in different orienting environments. Film and flow‐oriented FD‐LD spectra were collected down to 170 nm. Even for flow‐oriented liposomes, we have data collected down to 210 nm. For strongly scattering samples, for example, liposomes, FD‐LD has the clear advantage that scattering is absent for the longer wavelength fluorescence photons. The collimated and smaller beam size of the synchrotron radiation also gives rise to sharper and more well‐defined features in the spectra.
- Subjects
LINEAR dichroism; SYNCHROTRON radiation; SYNCHROTRON radiation sources; LIGHT scattering; PHOTON scattering; SYNCHROTRONS; FLUORESCENCE yield; FLUOROPHORES; LIGHT sources
- Publication
Chirality, 2024, Vol 36, Issue 4, p1
- ISSN
0899-0042
- Publication type
Article
- DOI
10.1002/chir.23667