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- Title
Performance evaluation of a high‐throughput separation system for circulating tumor cells based on microcavity array.
- Authors
Negishi, Ryo; Saito, Hyuga; Iwata, Reito; Tanaka, Tsuyoshi; Yoshino, Tomoko
- Abstract
Circulating tumor cells (CTCs) are widely known as useful biomarkers in the liquid biopsies of cancer patients. Although single‐cell genetic analysis of CTCs is a promising diagnostic tool that can provide detailed clinical information for precision medicine, the capacity of single‐CTC isolation for genetic analysis requires improvement. To overcome this problem, we previously developed a multiple single‐cell encapsulation system for CTCs using hydrogel‐encapsulation, which allowed for the high‐throughput isolation of single CTCs. However, isolation of a single cell from adjacent cells remained difficult and often resulted in contamination by neighboring cells due to the limited resolution of the generated hydrogel. We developed a novel multiple single‐cell encapsulation system equipped with a high magnification lens for high throughput and a more accurate single‐cell encapsulation. The multiple single‐cell encapsulation system has sufficient sensitivity to detect immune‐stained CTCs, and could also generate a micro‐scaled hydrogel that can isolate a single cell from adjacent cells within 10 µm, with high efficiency. The proposed system enables high throughput and accurate single‐cell manipulation and genome amplification without contamination from neighboring cells.
- Subjects
CELL separation; INDIVIDUALIZED medicine; BIOMARKERS; CANCER patients
- Publication
Engineering in Life Sciences, 2020, Vol 20, Issue 11, p485
- ISSN
1618-0240
- Publication type
Article
- DOI
10.1002/elsc.202000024