We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Preparation, Characterization, and In Vitro Release of Curcumin-Loaded IRMOF-10 Nanoparticles and Investigation of Their Pro-Apoptotic Effects on Human Hepatoma HepG2 Cells.
- Authors
Yin, Dongge; Hu, Xueling; Cai, Mengru; Wang, Kaixin; Peng, Hulinyue; Bai, Jie; Xv, Yvchen; Fu, Tingting; Dong, Xiaoxv; Ni, Jian; Yin, Xingbin
- Abstract
Curcumin (CUR) has a bright future in the treatment of cancer as a natural active ingredient with great potential. However, curcumin has a low solubility, which limits its clinical application. In this study, IRMOF-10 was created by the direct addition of triethylamine, CUR was loaded into IRMOF-10 using the solvent adsorption method, and the two were characterized using a scanning electron microscope (SEM), X-ray diffraction (XRD), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG) methods, and Brunauer–Emmett–Teller (BET) analysis. We also used the MTT method, 4′,6-diamidino-2-phenylindole (DAPI) staining, the annexin V/PI method, cellular uptake, reactive oxygen species (ROS), and the mitochondrial membrane potential (MMP) to perform a safety analysis and anticancer activity study of IRMOF-10 and CUR@IRMOF-10 on HepG2 cells. Our results showed that CUR@IRMOF-10 had a CUR load of 63.96%, with an obvious slow-release phenomenon. The CUR levels released under different conditions at 60 h were 33.58% (pH 7.4) and 31.86% (pH 5.5). Cell experiments proved that IRMOF-10 was biologically safe and could promote curcumin entering the nucleus, causing a series of reactions, such as an increase in reactive oxygen species and a decrease in the mitochondrial membrane potential, thereby leading to cell apoptosis. In summary, IRMOF-10 is an excellent drug carrier and CUR@IRMOF-10 is an effective anti-liver cancer sustained-release preparation.
- Subjects
FOURIER transform infrared spectroscopy; REACTIVE oxygen species; HEPATOCELLULAR carcinoma; SCANNING electron microscopes; MITOCHONDRIAL membranes; CONTROLLED release drugs; CONTROLLED release preparations
- Publication
Molecules, 2022, Vol 27, Issue 12, p3940
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27123940