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- Title
Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection.
- Authors
Qi Lei; Jing-Jing Hu; Lei Rong; Han Cheng; Yun-Xia Sun; Xian-Zheng Zhang
- Abstract
Endo/lysosomal escape and subsequent nuclear translocation are recognized as the two major challenges for efficient gene transfection. Herein, nuclear localization signal (NLS) peptide sequences and oligomeric lysine sequences were crosslinked via disulfide bonds to obtain glutathione (GSH) reducible polypeptide (pNLS). The pNLS could condense DNA into compact positive-charged complexes with redox sensitivity, and then gold nanoclusters (AuNC) were further decorated to the surface via electrostatic interactions obtaining versatile pNLS/DNA/AuNC complexes. The AuNC could generate reactive oxygen species (ROS) under NIR-irradiation and accelerate the endo/lysosomal escape of the complexes, and then the pNLS sequence degraded by GSH in cytoplasm would release the DNA and facilitate the subsequent nuclear translocation for enhanced gene transfection.
- Subjects
GENE transfection; DNA analysis; CHEMICAL synthesis; POLYPEPTIDES; GLUTATHIONE derivatives; OLIGOMERIC proanthocyanidins
- Publication
Molecules, 2016, Vol 21, Issue 8, p1103
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules21081103