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- Title
Clinical significance of miRNA-21, -103, -129, -150 in serous ovarian cancer.
- Authors
Wilczyński, Miłosz; Żytko, Ewelina; Danielska, Justyna; Szymańska, Bożena; Dzieniecka, Monika; Nowak, Marek; Malinowski, Jakub; Owczarek, Dariusz; Wilczyński, Jacek R.
- Abstract
<bold>Purpose: </bold>We aimed to compare expression levels of miRNA-21, -103, -129, -150 in primary tumour tissues and its omental metastases from patients operated for advanced ovarian serous cancer. Expression levels of selected miRNAs were correlated with clinicopathological features, including chemosensitivity and survival.<bold>Methods: </bold>We performed total RNA extraction from archival formalin-fixed paraffin-embedded tissue samples of primary serous ovarian cancer and omental metastases. The study included 48 patients with advanced ovarian cancer. The reference group consisted of 48 normal ovarian tissue samples. We performed cDNA synthesis, real time polymerase chain reaction and assessed relative expression of selected miRNAs.<bold>Results: </bold>Samples derived from serous ovarian cancer were characterized by higher expression levels of miRNA-150 in comparison to omental metastases (p = 0.045). Furthermore, we observed that shorter progression free-survival was associated with lower levels of miRNA-150 in metastatic tissues. We did not find similar relationships for other miRNAs.<bold>Conclusions: </bold>MiRNA-150 may potentially serve as a prognostic factor in advanced ovarian cancer. However, further studies are required to clearly confirm such hypothesis.
- Subjects
MICRORNA; OVARIAN cancer; OVARIAN diseases; METASTASIS; ANTISENSE DNA; RNA metabolism; COMPARATIVE studies; GENES; RESEARCH methodology; MEDICAL cooperation; OVARIAN tumors; POLYMERASE chain reaction; PROGNOSIS; RESEARCH; RESEARCH funding; RNA; SURVIVAL; TUMORS; TUMOR classification; EVALUATION research; CASE-control method
- Publication
Archives of Gynecology & Obstetrics, 2018, Vol 297, Issue 3, p741
- ISSN
0932-0067
- Publication type
journal article
- DOI
10.1007/s00404-018-4660-5