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- Title
A novel rat model of hip pain by intra-articular injection of nerve growth factor-characteristics of sensory innervation and inflammatory arthritis.
- Authors
Takanori Omae; Junichi Nakamura; Seiji Ohtori; Sumihisa Orita; Kazuyo Yamauchi; Shuichi Miyamoto; Shigeo Hagiwara; Shunji Kishida; Kazuhisa Takahashi
- Abstract
Objectives. To determine the direct effects of intra-articular injection of nerve growth factor (NGF) into normal rat hips and the time course of pain-related mediator appearance. Methods. Using 36 numbers of 8-week-old male Sprague-Dawley rats, 30 ml of 1% Fluoro-Gold solution (FG) (Sham-operated group; n = 12), 30 ml of 1% FG with 50 mg/ml NGF (NGF50 group; n = 12), and 30 ml of 1% FG with 100 mg/ml NGF (NGF100 group; n = 12) were injected into the left hip joints. Neurons in the dorsal root ganglion (DRG) labeled with FG, and FG and calcitonin gene-related peptide-immunoreactivity (CGRP-IR) were counted. The synovia in the left hip joint was examined histologically. Results. The NGF50 and NGF100 groups showed evidence of synovitis without cartilage degeneration compared with the Sham-operated group. At 7 days, the proportions of CGRP-IR FG-labeled to total FG-labeled neurons were 12%, 18%, and 36% in the Sham-operated, NGF50, and NGF100 groups, respectively. At 14 days, the proportions were 13%, 22%, and 35% in the Shamoperated, NGF50, and NGF100 groups, respectively. At 7 and 14 days, the NGF50 and NGF100 groups showed a significantly higher proportion of CGRP-IR FG-labeled neurons than the Shamoperated group. Conclusions. Intra-articular administration of NGF into the hip joint produces a novel rat model for hip pain.
- Subjects
NERVE growth factor; CALCITONIN gene-related peptide; NEURONS; HIP joint diseases; PAIN management
- Publication
Modern Rheumatology, 2015, Vol 25, Issue 6, p931
- ISSN
1439-7595
- Publication type
Article
- DOI
10.3109/14397595.2015.1023977