We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
High-fat diet alters the oligosaccharide chains of colon mucins in mice.
- Authors
Mastrodonato, Maria; Mentino, Donatella; Portincasa, Piero; Calamita, Giuseppe; Liquori, Giuseppa; Ferri, Domenico
- Abstract
Mucins are high molecular weight epithelial proteins, strongly glycosylated, and are the main component of the mucus. Since mucus secretion can be altered in diseases, colon mucins can be regarded as a biomarker of chronic inflammatory bowel diseases or preneoplastic changes. Conventional histochemistry and lectin histochemistry combined with chemical treatment and enzymatic digestion were carried out to analyze the colon mucins in mice fed a high-fat diet for 25 weeks, a period sufficient to induce simple liver steatosis, to check whether the carbohydrate features of mucus can be altered by an inadequate diet. An increase in the sialo/sulfomucins ratio with respect to control mice, assessed by computerized image analysis, was observed in the colon, although differences in sialic acid acetylation between control and mice fed a high-fat diet were not found. High-fat diet was also associated with altered lectin-binding pattern of the mucus, with a probable shortening of oligosaccharide chains of glycoproteins. This pattern was leading to over-expression of Galβ1,3GalNAc terminal dimers (TF antigen) and GalNAc terminal residues (Tn antigen). This altered composition of mucins can be related to a defect in the process of glycosylation, or to incomplete maturation of goblet cells, and may be an early indication of preneoplastic and neoplastic changes. In conclusion, our findings confirm that a fatty-rich diet (Western-style diet) induces alteration of mucins and may be associated with colon diseases. Our investigation corroborates the usefulness of lectins histochemistry in the early diagnosis of prepathological states of the colon.
- Subjects
OLIGOSACCHARIDES; MUCINS; MOLECULAR weights; MUCUS; BIOMARKERS; INFLAMMATORY bowel diseases; LABORATORY mice
- Publication
Histochemistry & Cell Biology, 2014, Vol 142, Issue 4, p449
- ISSN
0948-6143
- Publication type
Article
- DOI
10.1007/s00418-014-1221-2