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- Title
The endocytic pathway followed by the keratinocyte growth factor receptor.
- Authors
Belleudi, Francesca; Ceridono, Mara; Capone, Alessandro; Serafino, Annalucia; Marchese, Cinzia; Picardo, Mauro; Frati, Luigi; Torrisi, Maria Rosaria
- Abstract
Keratinocyte growth factor (KGF/FGF7) acts specifically on epithelial cells and regulates their proliferation and differentiation. It binds to and activates a receptor tyrosine kinase, the KGF receptor (KGFR), which is a splicing variant of the fibroblast growth factor receptor 2. The endocytic pathway followed by KGF and its receptor was analyzed here using immunofluorescence and confocal microscopy. After 10 min of internalization at 37°C, both KGF and its receptor were localized in early endosomes, and after 30–60 min of endocytosis ligand and receptor were seen to reach perinuclear late endosomes and not the recycling endosomal compartment. Parallel western blot analysis revealed that KGFRs were tyrosine phosphorylated both at early and late steps of internalization, suggesting that KGF and KGFR remain associated in active complexes through the endocytic pathway. Pulse-chase experiments showed that the internalized KGFRs underwent degradation detectable at 1 h of endocytosis at 37°C, indicating that KGFRs are functionally downregulated.
- Subjects
KERATINOCYTES; GROWTH factors; EPITHELIAL cells; ENDOCYTOSIS; ABSORPTION (Physiology); CELL physiology; TYROSINE
- Publication
Histochemistry & Cell Biology, 2002, Vol 118, Issue 1, p1
- ISSN
0948-6143
- Publication type
Article
- DOI
10.1007/s00418-002-0424-0