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- Title
JunB is required for endothelial cell morphogenesis by regulating core-binding factor β.
- Authors
Licht, Alexander H.; Pein, Oliver T.; Florin, Lore; Hartenstein, Bettina; Angel, Peter; Schorpp-Kistner, Marina; Reuter, Hendrik; Lichter, Peter; Arnold, Bernd
- Abstract
The molecular mechanism triggering the organization of endothelial cells (ECs) in multicellular tubules is mechanistically still poorly understood. We demonstrate that cell-autonomous endothelial functions of the AP-1 subunit JunB are required for proper endothelial morphogenesis both in vivo in mouse embryos with endothelial-specific ablation of JunB and in in vitro angiogenesis models. By cDNA microarray analysis, we identified core-binding factor β (CBFβ), which together with the Runx proteins forms the heterodimeric core-binding transcription complex CBF, as a novel JunB target gene. In line with our findings, expression of the CBF target MMP-13 was impaired in JunB-deficient ECs. Reintroduction of CBFβ into JunB-deficient ECs rescued the tube formation defect and MMP-13 expression, indicating an important role for CBFβ in EC morphogenesis.
- Subjects
CELL morphology; MORPHOGENESIS; GROWTH factors; CELL growth; MORPHOLOGY
- Publication
Journal of Cell Biology, 2006, Vol 175, Issue 6, p981
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200605149