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- Title
Development of a gel formulation with human umbilical cord mesenchymal stem cell-derived exosomes (UC-MSC-Exo) for cosmetic applications.
- Authors
Li Ting Kee; Min Hwei Ng; Jhi Biau Foo; Chee Wun How; Jia Xian Law
- Abstract
Introduction: Functional cosmetics, which involves the collaboration of stem cell technology with cosmetics, is an emerging trend in the cosmetic industry. Various studies indicated that umbilical cord-derived mesenchymal stem cell (UC-MSC) conditioned media contained various growth factors and exosomes that promote wound closure and re-epithelialization. However, it is still unclear if the UC-MSC-derived exosomes can be used as a cosmetic ingredient to enhance skin rejuvenation. Therefore, the current study investigates UC-MSC-Exo effectiveness in stimulating skin ECM synthesis and formulated as a gel formulation for cosmetic applications. Methods: Exosomes will be isolated from UC-MSCs and characterized. Then, the cytotoxicity of exosomes on human skin fibroblasts will be determined through MTT assay and cell growth rate will be determined via trypan blue exclusion assay. The efficacy of UC-MSCExo in promoting extracellular matrix (ECM) protein will be investigated through real time-polymerase chain reaction (RT-PCR). Then, the penetration of UC-MSC-Exo into the skin will be determined using fluorescent microscopy. UCMSC- Exo gel formulation will be developed, and the stability of the formulation will be tested. Functional analysis of UC-MSC-Exo formulation for animal skin permeation and promoting extracellular matrix protein synthesis of human skin fibroblasts will be investigated using fluorescent microscopy and RT-PCR. Expected Results: UC-MSC-Exo will stimulate the ECM protein synthesis by dermal fibroblasts. The optimal concentration of UC-MSC-Exo will be used to prepare as a formulation in the gel form for cosmetic applications. UC-MSC-Exo-based formulation shall be safe, stable and effective in stimulating ECM protein synthesis. Conclusion: UC-MSC-Exo is expected to stimulate skin ECM synthesis and formulated as a formulation for cosmetic applications. UC-MSC-Exo may have the potential to be used as a cosmetic ingredient in cosmeceuticals to promote skin rejuvenation.
- Subjects
UMBILICAL cord; EXTRACELLULAR matrix proteins; EXOSOMES; MESENCHYMAL stem cells; EXTRACELLULAR matrix
- Publication
Malaysian Journal of Medicine & Health Sciences, 2022, Vol 18, p23
- ISSN
1675-8544
- Publication type
Article