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- Title
Viability assay of coralline algae using triphenyltetrazolium chloride.
- Authors
Sun-Mee Park; Se-Eun Kang; Jae-Suk Choi; Ji-Young Cho; Seung-Je Yoon; Dong-Hyun Ahn; Yong-Ki Hong
- Abstract
The article examines the viability assay of coralline algae using triohenyltetrazolium chloride. Several techniques for determining the viability assay were hypothesized however none of these was proven reliable. These include counting numbers of stained cells under the microscope and analysis on the color of the algae. In its aimed to determine the viability and proliferation, the authors have optimized conditions of the tetrazolium chloride (TTC) assay, removing phycoerythrin or byproducts, for use in the viability determination of coralline algae. Optimization of conditions for the TTC viability assay was required with respect to pH of the incubation buffer, time of incubation, concentration of TTC, and temperature of incubation.
- Subjects
CORALLINE algae; MARINE algae; TETRAZOLIUM chloride; GROWTH of plant cells &; tissues; PLANT growth; FISHERY sciences; AQUATIC sciences; FEASIBILITY studies; RESEARCH
- Publication
Fisheries Science, 2006, Vol 72, Issue 4, p912
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1111/j.1444-2906.2006.01237.x