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- Title
Timosaponin B-II Ameliorates Palmitate-Induced Insulin Resistance and Inflammation via IRS-1/PI3K/Akt and IKK/NF-B Pathways.
- Authors
Yuan, Yong-Liang; Lin, Bao-Qin; Zhang, Chun-Feng; Cui, Ling-Ling; Ruan, Shi-Xia; Yang, Zhong-Lin; Li, Fei; Ji, De
- Abstract
This study aimed to investigate the effect of timosaponin B-II (TB-II) on palmitate (PA)-induced insulin resistance and inflammation in HepG2 cells, and probe the potential mechanisms. TB-II, a main ingredient of the traditional Chinese medicine Anemarrhena asphodeloides Bunge, notably ameliorated PA-induced insulin resistance and inflammation, and significantly improved cell viability, decreased PA-induced production of tumor necrosis factor- (TNF-) and interleukin-6 (IL-6) levels. Further, TB-II treatment notably decreased malondialdehyde (MDA) and lactate dehydrogenase (LDH) levels, and improved superoxide dismutase (SOD) and nitric oxide (NO). TB-II also reduced HepG2 cells apoptosis. Insulin receptor substrate-1 (IRS1)/phosphatidylinositol 3-kinase (PI3K)/Akt and inhibitor of nuclear factor -B kinase (IKK)/NF-B pathways-related proteins, and IKK, p65 phosphorylation, serine phosphorylation of insulin receptor substrate-1 (IRS-1) at S307, tyrosine phosphorylation of IRS-1, and Akt activation were determined by Western blot. Compared to model group, TB-II significantly downregulated the expression of p-NF-Bp65, p-IKK, p-IRS-1, p-PI3K and p-Akt. TB-II is a promising potential agent for the management of palmitate-induced insulin resistance and inflammation, which might be via IR/IRS-1/PI3K/Akt and IKK/NF-B pathways.
- Subjects
ANALYSIS of variance; APOPTOSIS; BIOLOGICAL assay; CELL culture; INFLAMMATION; INSULIN resistance; INTERLEUKINS; MEDICINAL plants; RESEARCH funding; STATISTICS; TUMOR necrosis factors; WESTERN immunoblotting; PHYTOCHEMICALS; DATA analysis; TREATMENT effectiveness; DATA analysis software
- Publication
American Journal of Chinese Medicine, 2016, Vol 44, Issue 4, p755
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X16500415