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- Title
Antennal transcriptome analysis of chemosensory genes in the cowpea beetle, Callosobruchus maculatus (F.).
- Authors
Tanaka, Keisuke; Shimomura, Kenji; Hosoi, Akito; Sato, Yui; Oikawa, Yukari; Seino, Yuma; Kuribara, Takuto; Yajima, Shunsuke; Tomizawa, Motohiro
- Abstract
Olfaction, one of the most important sensory systems governing insect behavior, is a possible target for pest management. Therefore, in this study, we analyzed the antennal transcriptome of the cowpea beetle, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchinae), which is a major pest of stored pulses and legumes. The de novo antennal RNA-seq assembly results identified 17 odorant, 2 gustatory, and 10 ionotropic receptors, 1 sensory neuron membrane protein, and 12 odorant-binding and 7 chemosensory proteins. Moreover, differential gene expression analysis of virgin male and female antennal samples followed by qRT-PCR revealed 1 upregulated and 4 downregulated odorant receptors in males. We also performed homology searches using the coding sequences built from previously proposed amino acid sequences derived from genomic data and identified additional chemosensory-related genes.
- Subjects
COWPEA weevil; COWPEA; CHEMOSENSORY proteins; BEETLES; AMINO acid sequence; OLFACTORY receptors
- Publication
PLoS ONE, 2022, Vol 17, Issue 1, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0262817