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- Title
Mobilization of P[EP)EP3313 Artificial Transposon in the Germilne of Drosopizila melanogaster.
- Authors
Graur, Marian; Ratiu, Attila C.; Ecovoiu, Alexandru Al.; Savu, Lorand
- Abstract
One of the most efficient and versatile mutagenesis methods used in Drosophila melanogaster (the fruit fly) relies on the mobilization of artificial derivates of P transposon. Aiming to reveal the biological significance of the patterns of transposition specific to P mobile element, mobilization of P{EP} artificial derivative was induced with a Δ2-3 source in both male and female germline of D. melanogaster. By transposition of the viable and fertile insertion P{EP}3313, located in 68B1 at 592 pb downstream from 3′UTR of CG6199 gene, 46 new transgenic strains were constructed, each of them containing diverse reinsetions of P{EP). Here we describe a combination of genetic and molecular experiments aiming to detect if conservation of the original insertion P{EP}EP3313 is associated with reinsertions in these mutant lines. A triplex PCR screening identified 22 mutant lines with conservation of P{EP}EP3313, a figure standing for 47,83 % of the transposition phenomena taken into account.
- Subjects
MOBILE genetic elements; DROSOPHILA; FRUIT flies; DROSOPHILA melanogaster; TRANSPOSONS; RADIOGENETICS
- Publication
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Animal Science & Biotechnologies, 2009, Vol 66, Issue 1/2, p441
- ISSN
1843-5262
- Publication type
Article