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- Title
Effects of Female Sex Hormones on Responses to CGRP, Acetylcholine, and 5-HT in Rat Isolated Arteries.
- Authors
Gupta, Saurabh; Mehrotra, Suneet; Villalón, Carlos; De Vries, Ren; Garrelds, Ingrid; Saxena, Pramod; VanDenBrink, Antoinette Maassen
- Abstract
Background.—Female sex hormones are implicated in the modulation of reactivity of a wide range of blood vessels under physiological as well as pathological conditions. Migraine, a neurovascular syndrome, is 3 times more prevalent in women during their reproductive period than in men. Objective.—This study sets out to investigate the effects of the female sex steroids, 17β-estradiol and progesterone (separately and in combination) on vasoactive responses to calcitonin gene-related peptide (CGRP), acetylcholine, and 5-hydroxytryptamine (5-HT) in rat isolated mesenteric, caudal, and basilar arteries. Methods.—Female Sprague-Dawley rats were ovariectomized (Day 0) and 7 days later subcutaneously implanted with pellets releasing over a 21-day period 17β-estradiol (0.25 mg), progesterone (50 mg), their combination, or placebo. On days 25-28, the animals were killed, arteries isolated and mounted in Mulvany myographs, and cumulative concentration response curves to CGRP, acetylcholine, and 5-HT were constructed. Results.—The relaxant responses to CGRP were significantly potentiated in mesenteric and caudal arteries from rats treated with 17β-estradiol as compared to the placebo-treated rats. Acetylcholine-induced relaxations were potentiated in the caudal artery from rats treated with the combination of 17β-estradiol and progesterone, as compared to that from placebo-treated rats. The 5-HT-induced contractions in the 3 arteries were not significantly different in efficacy or potency. Conclusion.—Our results show that 17β-estradiol potentiates CGRP-induced relaxations in the mesenteric and caudal arteries, while the combination treatment enhances acetylcholine-induced relaxations in the caudal artery. Although these in vitro experiments have been carried out in rats and a direct extrapolation to migraine in humans is not possible, our results may provide a new avenue to study the effects of sex steroids on vascular reactivity.
- Subjects
MIGRAINE; HEADACHE; SEX hormones; PROGESTERONE; BLOOD vessels; ANIMAL experimentation
- Publication
Headache: The Journal of Head & Face Pain, 2007, Vol 47, Issue 4, p564
- ISSN
0017-8748
- Publication type
Article
- DOI
10.1111/j.1526-4610.2006.00596.x