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- Title
Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives.
- Authors
Lee, Suji; Sung, Dan-Bi; Kang, Seungyoon; Parameswaran, Saravanan; Choi, Jun-Ho; Lee, Jong Seok; Han, Min Su
- Abstract
The level of human serum albumin (HSA) in biological fluids is a key health indicator and its quantitative determination has great clinical importance. In this study, we developed a selective and sensitive fluorescent HSA probe by fluorescence-based high-throughput screening of a set of fluorescent thieno[3,2-b]pyridine-5(4H)-one derivatives against major plasma proteins: HSA, bovine serum albumin (BSA), globulin, fibrinogen, and transferrin. The fluorophore chosen finally (4) showed noticeable fluorescence enhancement in the presence of HSA (160-fold increase), and it exhibited rapid response, high sensitivity (detection limit 8 nM), and the ability to clearly distinguish HSA from BSA in pH 9 buffer condition. Moreover, the probe could be applicable to detect trace amounts of HSA in an artificial urine sample; further, it might be applied to the determination of the HSA concentration in complex biological samples for pre-clinical diagnosis.
- Subjects
FLUORESCENT probes; BLOOD proteins; SERUM albumin; INTRAMOLECULAR charge transfer; DETECTION limit; GLOBULINS
- Publication
Sensors (14248220), 2019, Vol 19, Issue 23, p5298
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s19235298