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- Title
Serum Amylases and their Inhibitors: 1. Clinical Observations.
- Authors
Tuzhilin, S. A.; Gonda, M.; Carbonell, G.; Dreiling, D. A.
- Abstract
The difference between the methods of amylase determination lies the use of different substrates and in the final product of reaction. There are three methods in use: <em>1)</em> amyloclastic method (determination of decreases in iodine-starch color); <em>2)</em> saccharogenic method (determination of increases of reducing substances); <em>3)</em> chromogenic method (splitting of semisynthetic substrates). Each method determines a certain set of amylolytic enzymes splitting glycogen or starch and differs from each other by the quantity of substrates being split, the quantity of the final product as well as the specific isoenzyme tested. Moreover, the groups of enzymes determined by the different methods differ in availability and properties of enzyme inhibitors. Amylolytic enzymes (pancreatic) determined by splitting semi-synthetic substrates have minimal inhibitors and are demonstrated by increases of serum amylase as seen in acute and exacerbation of chronic pancreatitis. Amylases possessing inhibitors may be of significance in diagnosing of chronic pancreatitis. Increase of amylase activity in blood indicates only the acuteness of the process but not the severity of the disease. Such increases can be observed as well in acute or active processes in the hepato-biliary tract, e.g., active liver cirrhosis, acute or exacerbation of chronic cholecystitis, and in patients after cholecystectomy. Two amylase inhibitors were determined in serum: in diluted serum (DAI) and in serum incubated with trypsin (TAI). Amylase inhibitors increase in patients with chronic pancreatitis. In exacerbation of pancreatitis the inhibitors are significantly depressed.
- Subjects
AMYLASE inhibitors; SERUM; PANCREATIC diseases; GLYCOGEN; STARCH; DIGESTIVE enzymes
- Publication
American Journal of Gastroenterology (Springer Nature), 1982, Vol 77, Issue 1, p18
- ISSN
0002-9270
- Publication type
Article