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- Title
Structural Basis of Arrestin Selectivity for Active Phosphorylated G Protein-Coupled Receptors.
- Authors
Karnam, Preethi C.; Vishnivetskiy, Sergey A.; Gurevich, Vsevolod V.
- Abstract
Arrestins are a small family of proteins that bind G protein-coupled receptors (GPCRs). Arrestin binds to active phosphorylated GPCRs with higher affinity than to all other functional forms of the receptor, including inactive phosphorylated and active unphosphorylated. The selectivity of arrestins suggests that they must have two sensors, which detect receptor-attached phosphates and the active receptor conformation independently. Simultaneous engagement of both sensors enables arrestin transition into a high-affinity receptor-binding state. This transition involves a global conformational rearrangement that brings additional elements of the arrestin molecule, including the middle loop, in contact with a GPCR, thereby stabilizing the complex. Here, we review structural and mutagenesis data that identify these two sensors and additional receptor-binding elements within the arrestin molecule. While most data were obtained with the arrestin-1-rhodopsin pair, the evidence suggests that all arrestins use similar mechanisms to achieve preferential binding to active phosphorylated GPCRs.
- Subjects
ARRESTINS; G protein coupled receptors; MUTAGENESIS; MOLECULES
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 22, p12481
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms222212481