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- Title
Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine.
- Authors
Aubert, Léa; Dubus, Marie; Rammal, Hassan; Bour, Camille; Mongaret, Céline; Boulagnon-Rombi, Camille; Garnotel, Roselyne; Schneider, Céline; Rahouadj, Rachid; Laurent, Cedric; Gangloff, Sophie C.; Velard, Frédéric; Mauprivez, Cedric; Kerdjoudj, Halima
- Abstract
Maintenance of mesenchymal stem cells (MSCs) requires a tissue-specific microenvironment (i.e., niche), which is poorly represented by the typical plastic substrate used for two-dimensional growth of MSCs in a tissue culture flask. The objective of this study was to address the potential use of collagen-based medical devices (HEMOCOLLAGENE® Saint-Maur-des-Fossés, France) as mimetic niche for MSCs with the ability to preserve human MSC stemness in vitro. With a chemical composition similar to type I collagen, HEMOCOLLAGENE® foam presented a porous and interconnected structure (>90%) and a relative low elastic modulus of around 60 kPa. Biological studies revealed an apparently inert microenvironment of HEMOCOLLAGENE® foam, where 80% of cultured human MSCs remained viable, adopted a flattened morphology, and maintained their undifferentiated state with basal secretory activity. Thus, three-dimensional HEMOCOLLAGENE®foams present an in vitro model that mimics the MSC niche with the capacity to support viable and quiescent MSCs within a low stiffness collagen I scaffold simulating Wharton's jelly. These results suggest that haemostatic foam may be a useful and versatile carrier for MSC transplantation for regenerative medicine applications.
- Subjects
REGENERATIVE medicine; STEM cells; COLLAGEN; MESENCHYMAL stem cells; BIOCOMPATIBILITY
- Publication
International Journal of Molecular Sciences, 2017, Vol 18, Issue 10, p2210
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms18102210