We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Cell-Penetrating Peptide as a Means of Directing the Differentiation of Induced Pluripotent Stem Cells.
- Authors
Taku Kaitsuka; Kazuhito Tomizawa
- Abstract
Protein transduction using cell-penetrating peptides (CPPs) is useful for the delivery of large protein molecules, including some transcription factors. This method is safer than gene transfection methods with a viral vector because there is no risk of genomic integration of the exogenous DNA. Recently, this method was reported as a means for the induction of induced pluripotent stem (iPS) cells, directing the differentiation into specific cell types and supporting gene editing/correction. Furthermore, we developed a direct differentiation method to obtain a pancreatic lineage from mouse and human pluripotent stem cells via the protein transduction of three transcription factors, Pdx1, NeuroD, and MafA. Here, we discuss the possibility of using CPPs as a means of directing the differentiation of iPS cells and other stem cell technologies.
- Subjects
CELL-penetrating peptides; DNA analysis; CELL analysis; LABORATORY mice; GENE transfection; PROTEIN analysis
- Publication
International Journal of Molecular Sciences, 2015, Vol 16, Issue 11, p26667
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms161125986