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- Title
Astragaloside IV Ameliorates Airway Inflammation in an Established Murine Model of Asthma by Inhibiting the mTORC1 Signaling Pathway.
- Authors
Jin, Hualiang; Wang, Limin; Li, Bei; Cai, Cui; Ye, Jian; Xia, Junbo; Ma, Shenglin
- Abstract
Astragaloside IV (AS-IV), a main active constituent of Astragalus membranaceus, has been confirmed to have antiasthmatic effects. However, it remained unclear whether the beneficial effects of AS-IV on asthma were attributed to the mTOR inhibition; this issue was the focus of the present work. BALB/c mice were sensitized and challenged with ovalbumin followed with 3 weeks of rest/recovery and then reexposure to ovalbumin. AS-IV was administrated during the time of rest and reexposure. The characteristic features of allergic asthma, including airway hyperreactivity, histopathology, cytokines (IL-4, IL-5, IL-13, IL-17, and INF-γ), and CD4+CD25+Foxp3+Treg cells in bronchoalveolar lavage fluid (BALF), and downstream proteins of mTORC1/2 signaling were examined. AS-IV markedly suppressed airway hyperresponsiveness and reduced IL-4, IL-5, and IL-17 levels and increased INF-γ levels in the BALF. Histological studies showed that AS-IV markedly decreased inflammatory infiltration in the lung tissues. Notably, AS-IV inhibited mTORC1 activity, whereas it had limited effects on mTORC2, as assessed by phosphorylation of mTORC1 and mTORC2 substrates S6 ribosomal protein, p70 S6 Kinase, and Akt, respectively. CD4+CD25+Foxp3+Treg cells in BALF were not significantly changed by AS-IV. Together, these results suggest that the antiasthmatic effects of AS-IV were at least partially from inhibiting the mTORC1 signaling pathway.
- Subjects
ASTHMA prevention; INFLAMMATION prevention; ANIMAL experimentation; ASTRAGALUS (Plants); BRONCHOALVEOLAR lavage; CELLULAR signal transduction; CYTOKINES; GROWTH factors; HISTOLOGY; INTERLEUKINS; INTRAVENOUS therapy; MICE; RESPIRATORY allergy; T cells; PLANT extracts
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2017, Vol 2017, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2017/4037086