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- Title
Human platelet antigen genotyping of platelet donors in southern Brazil.
- Authors
Merzoni, J.; Fagundes, I. S.; Lunardi, L. W.; Lindenau, J. D.‐R.; Gil, B. C.; Jobim, M.; Dias, V. G.; Merzoni, L.; Sekine, L.; Onsten, T. G. H.; Jobim, L. F.
- Abstract
Human platelet antigens (HPA) are immunogenic structures that result from single nucleotide polymorphisms ( SNPs) leading to single amino acid substitutions. This study sought to determine the allele and genotype frequencies of HPA-1, HPA-2, HPA-3, HPA-4, HPA-5 and HPA-15 in platelet donors from the state of Rio Grande do Sul ( RS), Brazil, and compare their allele frequencies to those observed in other populations. HPA genotyping was performed by PCR- SSP method. The study sample comprised 201 platelet donors (167 Caucasians and 34 non-Caucasians). Allele 'a' was that most commonly found for HPA-1 to 5 in both groups. The HPA-15ab genotype predominated over homozygous genotypes of this system. Fisher's exact test revealed statistically significant differences for the HPA-5 system, with a greater prevalence of the HPA-5b allele in non-Caucasians. The neighbour-joining method and principal components analysis revealed genetic proximity between our Caucasian group and European populations. We conclude that the allele frequencies of HPA-1 to 5 and HPA-15 found in our Caucasian sample are similar to those reported for European populations. These findings corroborate the ethnic makeup of the population of RS. The higher frequency of the HPA-5b allele found in the non-Caucasian group of our sample suggests the possibility of allosensitization in patients who receive platelet transfusions from genetically incompatible donors.
- Subjects
ANTIGENS; BLOOD platelets; SINGLE nucleotide polymorphisms; BLOOD platelet transfusion; CAUCASIAN race; PRINCIPAL components analysis; IMMUNOGENETICS
- Publication
International Journal of Immunogenetics, 2015, Vol 42, Issue 5, p329
- ISSN
1744-3121
- Publication type
Article
- DOI
10.1111/iji.12220