We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Enhanced low-cost lipopeptide biosurfactant production by Bacillus velezensis from residual glycerin.
- Authors
Brito, Henrique A.; Napp, Amanda P.; Pereira, Evandro; Bach, Evelise; Borowski, João V. B.; Passaglia, Luciane M. P.; Melo, Vania M. M.; Moreira, Raphael; Foster, E. Johan; Lopes, Fernanda C.; Vainstein, Marilene H.
- Abstract
Biosurfactants (BSFs) are molecules produced by microorganisms from various carbon sources, with applications in bioremediation and petroleum recovery. However, the production cost limits large-scale applications. This study optimized BSFs production by Bacillus velezensis (strain MO13) using residual glycerin as a substrate. The spherical quadratic central composite design (CCD) model was used to standardize carbon source concentration (30 g/L), temperature (34 °C), pH (7.2), stirring (239 rpm), and aeration (0.775 vvm) in a 5‐L bioreactor. Maximum BSFs production reached 1527.6 mg/L of surfactins and 176.88 mg/L of iturins, a threefold increase through optimization. Microbial development, substrate consumption, concentration of BSFs, and surface tension were also evaluated on the bioprocess dynamics. Mass spectrometry Q-TOF-MS identified five surfactin and two iturin isoforms produced by B. velezensis MO13. This study demonstrates significant progress on BSF production using industrial waste as a microbial substrate, surpassing reported concentrations in the literature.
- Subjects
SURFACE tension; SURFACTIN; BACILLUS (Bacteria); INDUSTRIAL wastes; FACTORIAL experiment designs; BIOSURFACTANTS
- Publication
Bioprocess & Biosystems Engineering, 2024, Vol 47, Issue 9, p1555
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-024-03051-y