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- Title
Intrinsically determined cell death of developing cortical interneurons.
- Authors
Southwell, Derek G.; Paredes, Mercedes F.; Galvao, Rui P.; Jones, Daniel L.; Froemke, Robert C.; Sebe, Joy Y.; Alfaro-Cervello, Clara; Tang, Yunshuo; Garcia-Verdugo, Jose M.; Rubenstein, John L.; Baraban, Scott C.; Alvarez-Buylla, Arturo
- Abstract
Cortical inhibitory circuits are formed by ?-aminobutyric acid (GABA)-secreting interneurons, a cell population that originates far from the cerebral cortex in the embryonic ventral forebrain. Given their distant developmental origins, it is intriguing how the number of cortical interneurons is ultimately determined. One possibility, suggested by the neurotrophic hypothesis, is that cortical interneurons are overproduced, and then after their migration into cortex the excess interneurons are eliminated through a competition for extrinsically derived trophic signals. Here we characterize the developmental cell death of mouse cortical interneurons in vivo, in vitro and after transplantation. We found that 40% of developing cortical interneurons were eliminated through Bax (Bcl-2-associated X)-dependent apoptosis during postnatal life. When cultured in vitro or transplanted into the cortex, interneuron precursors died at a cellular age similar to that at which endogenous interneurons died during normal development. Over transplant sizes that varied 200-fold, a constant fraction of the transplanted population underwent cell death. The death of transplanted neurons was not affected by the cell-autonomous disruption of TrkB (tropomyosin kinase receptor B), the main neurotrophin receptor expressed by neurons of the central nervous system. Transplantation expanded the cortical interneuron population by up to 35%, but the frequency of inhibitory synaptic events did not scale with the number of transplanted interneurons. Taken together, our findings indicate that interneuron cell death is determined intrinsically, either cell-autonomously or through a population-autonomous competition for survival signals derived from other interneurons.
- Subjects
CELL death; NEURAL circuitry; INTERNEURONS; PROSENCEPHALON; CEREBRAL cortex; TROPOMYOSINS; LABORATORY mice; PHYSIOLOGY
- Publication
Nature, 2012, Vol 491, Issue 7422, p109
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature11523