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- Title
A rapid visual detection method for Potato virus Y based on loop mediated isothermal amplification (LAMP).
- Authors
Ma, Yongqiang; Zhang, Yongpeng; Zhu, Haixia; Wang, Jian
- Abstract
In order to establish a rapid and sensitive LAMP visual detection method for Potato virus Y on-site, this study used the conserved fragment of PVY CP gene sequence as a template to design primers, and the rapid visual LAMP detection method of Potato virus Y was successfully established by optimizing the reaction system components (concentration ratio of internal and external primers, and concentrations of loop primers, Bst DNA, Mg2+, dNTP and betaine) and reaction conditions (temperature). The established rapid visual LAMP detection method for Potato virus Y can specifically identify Potato virus Y, providing complete visual detection within 40 min, and the detection limit can reach 4.34 pg μL−1 (cDNA) concentration, which is 100 times more sensitive than PCR. An analysis of 50 potato field samples showed that the results of rapid visualization LAMP and RT-PCR were basically the same. This method is suitable for the rapid detection of Potato virus Y on-site, and this study can also provide theoretical reference for the early diagnosis of other potato viruses.
- Subjects
POTATO virus Y; LOOP-mediated isothermal amplification; DETECTION limit
- Publication
Journal of Plant Diseases & Protection, 2023, Vol 130, Issue 6, p1423
- ISSN
1861-3829
- Publication type
Article
- DOI
10.1007/s41348-023-00786-w