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- Title
The Continuous Culture of Excised Rye Roots.
- Authors
Roberts, E. H.; Street, H. E.
- Abstract
1. The growth of excised roots of Petkus II rye in modified White's medium is stimulated by addition of B.D.H. Peptone or Difco Yeast Extract; the most marked stimulation is in lateral growth. Eighty per cent of the growth stimulation by the yeast extract arises from its content of L-tryptophane. 2. A clone of excised rye roots was established as sector cultures and maintained without diminution in growth rate through 38 passages of 14 days duration in medium supplement with 30 mg./l. Difco Yeast Extract. 3. The addition of yeast extract or L-tryptophane make possible the continued growth through successive passages of the main axis meristems of the clonal roots. 4. Nicotinic acid, L-Kynurenine, gramine and skatole did not stimulate growth. IAA and IAN at 1/400th the optimum molarity of tryptophane were equal to it in growth-promoting activity. D- and L-tryptophane bad the same activity. 5. Tryptophane added aseptically to previously autoclaved medium does not stimulate growth. The tryptophane is 'activated' during autoclaving or when refluxed in distilled water. 6 When medium containing 'activated' tryptophane is shaken with other. the partition of activity between ether and medium does not correspond with that which would be expected if activation is due to formation of IAA and/or IAN from the tryptophane during heating. Examination of 'activated' tryptophane by chromatography also supports the view that IAA formation does not account for its activity. 7. The culture technique described only makes possible the establishment of clones from about 10 per cent of tho grains of the rye sample used and fails to establish excised-root clones from the other cereals tested.
- Subjects
PLANT growing media; PLANT root physiology; PLANT nutrition; PLANT roots; PLANT physiology
- Publication
Physiologia Plantarum, 1955, Vol 8, Issue 2, p238
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1955.tb08974.x