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- Title
Utilization of host-derived cysteine-containing peptides overcomes the restricted sulphur metabolism of C ampylobacter jejuni.
- Authors
Vorwerk, Hanne; Mohr, Juliane; Huber, Claudia; Wensel, Olga; Schmidt‐Hohagen, Kerstin; Gripp, Eugenia; Josenhans, Christine; Schomburg, Dietmar; Eisenreich, Wolfgang; Hofreuter, Dirk
- Abstract
The non-glycolytic food-borne pathogen C ampylobacter jejuni successfully colonizes the intestine of various hosts in spite of its restricted metabolic properties. While several amino acids are known to be used by C . jejuni as energy sources, none of these have been found to be essential for growth. Here we demonstrated through phenotype microarray analysis that cysteine utilization increases the metabolic activity of C . jejuni. Furthermore, cysteine was crucial for its growth as C . jejuni was unable to synthesize it from sulphate or methionine. Our study showed that C . jejuni compensates this limited anabolic capacity by utilizing sulphide, thiosulphate, glutathione and the dipeptides γ Glu- Cys, Cys- Gly and Gly- Cys as sulphur sources and cysteine precursors. A panel of C . jejuni mutants in putative peptidases and peptide transporters were generated and tested for their participation in the catabolism of the cysteine-containing peptides, and the predicted transporter protein CJJ81176_0236 was discovered to facilitate the growth with the dipeptide Cys- Gly, Ile- Arg and Ile- Trp. It was named C ampylobacter peptide transporter A ( CptA) and is the first representative of the oligopeptide transporter OPT family demonstrated to participate in the glutathione-derivative Cys- Gly catabolism in prokaryotes. Our study provides new insights into how host- and microbiota-derived substrates like sulphide, thiosulphate and short peptides are used by C . jejuni to compensate its restricted metabolic capacities.
- Subjects
FOODBORNE diseases; CAMPYLOBACTER jejuni; BACTERIAL growth; HOST specificity (Biology); CYSTEINE metabolism
- Publication
Molecular Microbiology, 2014, Vol 93, Issue 6, p1224
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.12732