We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The novel globin protein fungoglobin is involved in low oxygen adaptation of A spergillus fumigatus.
- Authors
Hillmann, Falk; Linde, Jörg; Beckmann, Nicola; Cyrulies, Michael; Strassburger, Maria; Heinekamp, Thorsten; Haas, Hubertus; Guthke, Reinhard; Kniemeyer, Olaf; Brakhage, Axel A.
- Abstract
The human pathogenic fungus A spergillus fumigatus normally lives as a soil saprophyte. Its environment includes poorly oxygenated substrates that also occur during tissue invasive growth of the fungus in the human host. Up to now, few cellular factors have been identified that allow the fungus to efficiently adapt its energy metabolism to hypoxia. Here, we cultivated A . fumigatus in an O2-controlled fermenter and analysed its responses to O2 limitation on a minute timescale. Transcriptome sequencing revealed several genes displaying a rapid and highly dynamic regulation. One of these genes was analysed in detail and found to encode fungoglobin, a previously uncharacterized member of the sensor globin protein family widely conserved in filamentous fungi. Besides low O2, iron limitation also induced transcription, but regulation was not entirely dependent on the two major transcription factors involved in adaptation to iron starvation and hypoxia, HapX and SrbA respectively. The protein was identified as a functional haemoglobin, as binding of this cofactor was detected for the recombinant protein. Gene deletion in A . fumigatus confirmed that haem-binding fungoglobins are important for growth in microaerobic environments with O2 levels far lower than in hypoxic human tissue.
- Subjects
GLOBIN; HEMOGLOBINS; PROTEINS; BIOMOLECULES; ASPERGILLUS fumigatus
- Publication
Molecular Microbiology, 2014, Vol 93, Issue 3, p539
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.12679