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- Title
Sulforaphene and sulforaphane in commonly consumed cruciferous plants contributed to antiproliferation in HCT116 colon cancer cells.
- Authors
Pocasap, Piman; Weerapreeyakul, Natthida
- Abstract
Objective To analyze two isothiocyanates (sulforaphene and sulforaphane) and their antiproliferative effect of 11 indigenous cruciferous vegetables. Methods Phytoconstituents identification was conducted by high performance liquid chromatography and gas chromatography-mass spectrometer techniques. The antiproliferation was evaluated in colon cancer cell line HCT116 by MTT assay. Results Isothiocyanate identification by high performance liquid chromatography showed that broccoli, cabbage, “Khi-Hood” ( Raphanus sativus L. var. caudatus Alef) and Chinese radish contained isothiocyanates sulforaphane. Sulforaphene and sulforaphane in broccoli, cabbage and “Khi-Hood” were characterized by the gas chromatography-mass spectrometer analysis. Antiproliferation screening by MTT assay found that the potent plants which possessed IC 50 below 50 μg/mL were cabbage and “Khi-Hood”, while the others had low antiproliferation with IC 50 higher than 50 μg/mL. Difference in antiproliferation was probably due to difference existed phytochemical constituents in each plant. “Khi-Hood” possessed the highest antiproliferation against HCT116 with the lowest IC 50 at (9.42 ± 0.46) μg/mL. The IC 50 of chemotherapeutic drug (mitomycin C) was (19.12 ± 1.00) μg/mL, while both melphalan and 5-fluorouracil possessed the IC 50 value higher than 50 μg/mL. Conclusions Commonly consumed cruciferous vegetables exerted varied antiproliferation and isothiocyanate contents. High isothiocyanate content in “Khi-Hood” was contributed to high antiproliferation. Among 11 plants studied, “Khi-Hood” could be an alternative chemopreventive diet.
- Subjects
COLON cancer treatment; CANCER cell proliferation; BRASSICACEAE; SULFORAPHANE; CANCER chemotherapy; GAS chromatography/Mass spectrometry (GC-MS); HIGH performance liquid chromatography; THERAPEUTICS
- Publication
Asian Pacific Journal of Tropical Biomedicine, 2016, Vol 6, Issue 2, p119
- ISSN
2221-1691
- Publication type
Article
- DOI
10.1016/j.apjtb.2015.11.003