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- Title
Effects of Antisense Transforming Growth Factor-Β1 Gene Transfer on the Biological Activities of Tendon Sheath Fibroblasts.
- Authors
Changsuo Xia; Changrong Ding; Xuanying Yang; Kang Sun; Shaoqi Tian
- Abstract
Recent studies have shown the importance of transforming growth factor-beta (TGF-) in flexor tendon wound healing. Decreased adhesion formation and increased range of motion after the administration of TGF- antibodies after tendon repair have been shown. But TGF- antibodies have a short biologic half-life, and continuous supplementation of exogenous TGF- antibodies is not practical. Transfer of growth factor genes to tenocytes provides an alternative to protein therapeutics, and a gene therapy approach will prolong the availability of therapeutic proteins. We investigated the biological activities effects of rabbit tendon sheath fibroblasts transfected by antisense TGF-1 gene. Tendon sheath fibroblasts were isolated from New Zealand white rabbits and transfected by antisense TGF-1 gene with Lipofectin (Invitrogen, Carlsbad, California). Reverse transcription polymerase chain reaction was used to measure collagen I, collagen III, and TGF-1 expression, and Western blot was used to measure collagen protein I expression in tendon sheath fibroblasts after being transfected by antisense TGF-1 gene. Reverse transcription polymerase chain reaction displayed that tendon sheath fibroblasts transfected with antisense TGF-1gene showed marked decrease collagen I, collagen III, and TGF-1 mRNA expression. Western blot showed that tendon sheath fibroblasts transfected with antisense TGF-1 gene showed marked decrease expression of collagen I protein, and there was significant difference compared with the untransfected and empty transfected groups (P.01). Tendon sheath fibroblasts can transfect with antisense TGF-1 gene successfully and can decrease production of collagen I, collagen III, and TGF-1, which were factors of tendon adhere formation.
- Publication
Orthopedics, 2010, Vol 33, Issue 8, p1
- ISSN
0147-7447
- Publication type
Article
- DOI
10.3928/01477447-20100625-06