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- Title
Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis.
- Authors
Andersen-Ranberg, Johan; Kongstad, Kenneth Thermann; Nielsen, Morten Thrane; Jensen, Niels Bjerg; Pateraki, Irini; Bach, Søren Spanner; Hamberger, Britta; Zerbe, Philipp; Staerk, Dan; Bohlmann, Jörg; Møller, Birger Lindberg; Hamberger, Björn
- Abstract
Plant-derived diterpenoids serve as important pharmaceuticals, food additives, and fragrances, yet their low natural abundance and high structural complexity limits their broader industrial utilization. By mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional combinations of class I and II diterpene synthases, 41 of which are 'new-to-nature'. Stereoselective biosynthesis of over 50 diterpene skeletons was demonstrated, including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four industrially relevant targets was accomplished in engineered strains of Saccharomyces cerevisiae.
- Subjects
DITERPENES synthesis; BIOCATALYSIS; STEREOSELECTIVE reactions; STEREOCHEMISTRY; SACCHAROMYCES cerevisiae; COMBINATORIAL chemistry
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 6, p2142
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201510650