We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A Highly Selective Mitochondria-Targeting Fluorescent K<sup>+</sup> Sensor.
- Authors
Kong, Xiangxing; Su, Fengyu; Zhang, Liqiang; Yaron, Jordan; Lee, Fred; Shi, Zhengwei; Tian, Yanqing; Meldrum, Deirdre R.
- Abstract
Regulation of intracellular potassium (K+) concentration plays a key role in metabolic processes. So far, only a few intracellular K+ sensors have been developed. The highly selective fluorescent K+ sensor KS6 for monitoring K+ ion dynamics in mitochondria was produced by coupling triphenylphosphonium, borondipyrromethene (BODIPY), and triazacryptand (TAC). KS6 shows a good response to K+ in the range 30-500 m M, a large dynamic range ( Fmax/ F0≈130), high brightness ( ϕf=14.4 % at 150 m M of K+), and insensitivity to both pH in the range 5.5-9.0 and other metal ions under physiological conditions. Colocalization tests of KS6 with MitoTracker Green confirmed its predominant localization in the mitochondria of HeLa and U87MG cells. K+ efflux/influx in the mitochondria was observed upon stimulation with ionophores, nigericin, or ionomycin. KS6 is thus a highly selective semiquantitative K+ sensor suitable for the study of mitochondrial potassium flux in live cells.
- Subjects
MITOCHONDRIA; POTASSIUM channels; POTASSIUM ions; IONOPHORES; DRUG use testing
- Publication
Angewandte Chemie International Edition, 2015, Vol 54, Issue 41, p12053
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201506038