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- Title
FRECUENCIA, MORFOLOGÍA Y CONCENTRACIÓN DE PROGESTERONA in vitro DE CUERPOS LÚTEOS CAVITARIOS DE OVEJAS.
- Authors
Tolentino-Castro, Cristina; Miranda-Jiménez, Leonor; Quero-Carrillo, Adrián R.; Pescador-Salas, Nazario
- Abstract
The corpus luteum (CL) may develop as a compact or cavitary structure. But it is unknown whether the morphophysiological characteristics of compact corpora lutea (CLsCom) differ from that of cavitary corpora lutea (CLsCav), neither if these characteristics are determined by type, wool producing ewes (oveja de lana - OL) versus hair ewe (oveja de pelo - OP). Therefore, in our study ovaries were collected from sacrificed ewes and 81 corpora lutea were obtained from OL and 81 from OP. The experimental design was completely randomized with a 2X2 factorial arrangement of treatments. The variables evaluated were frequency, macroscopic and microscopic morphology, and production of progesterone (P4) in vitro, in 0.1 g luteum tissue, with or without addition of 350 IU of eCG. The frequency of CLsCav was higher (p⩽0.05) in OP (50.6 %) than in OL (35.8 %). The CLsCav were smaller (p⩽0.05) than the CLsCom. The P4 concentration in CLsCav and CLsCom of both types, with or without eCG, was similar (p>0.05) throughout culturing. The CLsCav from OP had fewer cells per visual field (19) and were larger (25 mm) than CLsCom cells (25.6 and 15 mm; p⩽0.05). Hair ewes had a higher percentage of CLsCav than wool ewes. The CLsCav were smaller than the CLCom and in vitro progesterone production of the two types of CL was similar.
- Publication
Agrociencia, 2015, Vol 49, Issue 3, p267
- ISSN
1405-3195
- Publication type
Article