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- Title
The MSL3 chromodomain directs a key targeting step for dosage compensation of the Drosophila melanogaster X chromosome.
- Authors
Sural, Tuba H.; Peng, Shouyong; Li, Bing; Workman, Jerry L.; Park, Peter J.; Kuroda, Mitzi I.
- Abstract
The male-specific lethal (MSL) complex upregulates the single male X chromosome to achieve dosage compensation in Drosophila melanogaster. We have proposed that MSL recognition of specific entry sites on the X is followed by local targeting of active genes marked by histone H3 trimethylation (H3K36me3). Here we analyze the role of the MSL3 chromodomain in the second targeting step. Using ChIP-chip analysis, we find that MSL3 chromodomain mutants retain binding to chromatin entry sites but show a clear disruption in the full pattern of MSL targeting in vivo, consistent with a loss of spreading. Furthermore, when compared to wild type, chromodomain mutants lack preferential affinity for nucleosomes containing H3K36me3 in vitro. Our results support a model in which activating complexes, similarly to their silencing counterparts, use the nucleosomal binding specificity of their respective chromodomains to spread from initiation sites to flanking chromatin.
- Subjects
DROSOPHILA melanogaster; FRUIT flies; GENETIC mutation; X chromosome; CHROMATIN; GENE expression
- Publication
Nature Structural & Molecular Biology, 2008, Vol 15, Issue 12, p1318
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.1520