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- Title
Actinomadura algeriensis sp. nov., an actinobacterium isolated from Saharan soil.
- Authors
Lahoum, Abdelhadi; Bouras, Noureddine; Mathieu, Florence; Schumann, Peter; Spröer, Cathrin; Klenk, Hans-Peter; Sabaou, Nasserdine
- Abstract
During the course of a screening programme for new taxa of actinobacteria, a strain designated ACD1, was isolated from a Saharan soil in the Hoggar region (Algeria). The taxonomic position of this strain was determined using a polyphasic taxonomic approach. The strain was observed to form extensively branched, non-fragmenting substrate mycelium, and aerial mycelium with straight to flexuous, hooked and irregular spirals (1-2 turns) forming short chains of spores. The diamino acid present in the cell wall is meso-diaminopimelic acid. Galactose, glucose, madurose, mannose and ribose occur in whole-cell hydrolysates. The diagnostic phospholipids detected were diphosphatidylglycerol and phosphatidylinositol. The major menaquinones were identified as MK-9 (H) and MK-9 (H). The major fatty acids were found to be C, C cis9, iso-C and 10-methyl C. Phylogenetic analysis based on the 16S rRNA gene showed that the strain belongs to the genus Actinomadura, and is closely related to Actinomadura sediminis DSM 45500 (98.5 % similarity) and Actinomadura cremea subsp. cremea DSM 43676 (98.3 % similarity). However, DNA-DNA hybridization revealed only 48.0 % relatedness with A. sediminis DSM 45500 and 33.2 % relatedness with A. cremea subsp. cremea DSM 43676. The combined phenotypic and genotypic data showed that the strain represents a novel species of the genus Actinomadura, for which the name Actinomadura algeriensis sp. nov. is proposed, with the type strain ACD1 (= DSM 46744 = CECT 8841).
- Subjects
AHAGGAR Mountains (Algeria); SAHARA; ACTINOMADURA; ACTINOBACTERIA; MYCELIUM; SOILS; ECOLOGY
- Publication
Antonie van Leeuwenhoek, 2016, Vol 109, Issue 1, p159
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-015-0617-x