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- Title
Optically sectioned in vivoimaging with speckle illumination HiLo microscopy.
- Authors
Daryl Lim; Tim N. Ford; Kengyeh K. Chu; Jerome Mertz
- Abstract
We present a simple wide-field imaging technique, called HiLo microscopy, that is capable of producing optically sectioned images in real time, comparable in quality to confocal laser scanning microscopy. The technique is based on the fusion of two raw images, one acquired with speckle illumination and another with standard uniform illumination. The fusion can be numerically adjusted, using a single parameter, to produce optically sectioned images of varying thicknesses with the same raw data. Direct comparison between our HiLo microscope and a commercial confocal laser scanning microscope is made on the basis of sectioning strength and imaging performance. Specifically, we show that HiLo and confocal 3-D imaging of a GFP-labeled mouse brain hippocampus are comparable in quality. Moreover, HiLo microscopy is capable of faster, near video rate imaging over larger fields of view than attainable with standard confocal microscopes. The goal of this paper is to advertise the simplicity, robustness, and versatility of HiLo microscopy, which we highlight with in vivoimaging of common model organisms including planaria, C. elegans, and zebrafish.
- Subjects
CEREBRAL cortex; SPECKLE interference; CONFOCAL microscopy; THREE-dimensional imaging; ROBUST control; ZEBRA danio; LABORATORY mice
- Publication
Journal of Biomedical Optics, 2011, Vol 16, Issue 1, p016014
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.3528656